Application:
CD61, clone NaM28-7D6, can be applied in flow cytometry for analysis of blood and bone marrow samples or in immuno-histochemistry using cytospots or frozen tissue sections
CD61 antibodies are applied in the diagnosis of Glanzmann thrombastemia and leukemia. CD41/CD61 is the major integrin on platelets and is important for platelet adhesion and aggregation. The ligands for CD41/CD61 include fibrinogen, von Willebrand factor, fibronectin and thrombospondin. Binding to these ligands depends on the activation state of the platelets
CD51/CD61 acts as an activation-independent receptor for platelet attachment and spreading on vitronectin. Other ligands include fibrinogen, fibronectin, von Willebrand factor, laminin and thrombospondin. CD51/CD61 may also mediate cell-cell adhesion via an interaction with CD31. Antagonists of CD51/CD61 have been shown to inhibit tumor growth by disrupting angiogenesis (3)

Specifications:
CD61, clone NaM28-7D6 produces mouse IgG1 immunoglobulins directed against a 105 kDa antigen. Clone NaM28-7D6 was clustered at the Leucocyte Typing Workshop VI (2).

Performance Characteristics:
CD61, clone NaM28-7D6 monoclonal antibodies were analyzed by flow cytometry using a preparation of concentrated thrombocytes. The cytogram shows direct staining with 10 ml of CD61FITC and 100 ml cell sample

Clone NaM28-7D6, CD61

Clone:	NaM28-7D6
Subclass:	IgG1
Source:	mouse
Cellular expression:	platelets, megakaryocytes, monocytes, macrophages and endothelial cells
Positive cells:	CD61 FITC 98.4%
Mean fluorescence units:	CD61 FITC 80.26

References:
1. Barclay A.N., et al (1997) The Leukocyte Antigen Factsbook. Academic Press. London.
2. Kishimoto, et al., 1996. Leukocyte Typing VI. Kobe.
3. Ginsberg,M.H., et al., 1993. Thromb.Haemost. 70. 87-93

For research only. Not for use in humans. For in vitro use only.