Application:
Monoclonal antibodies CD54, clone B-H17 can be applied in biological studies, flow cytometry, or in immuno-histochemistry using cytospots or frozen tissue sections.
Human CD54 is closely related structurally and functionally to CD50 (ICAM-3) and CD102 (ICAM-2) (4). Endothelial CD54 contributes to the extravasation of leucocytes from blood vessels, particularly in areas of inflammation (5). CD54 on antigen-presenting cells contributes to antigen-specific T cell activation, presumably by enhancing interactions between T cells and antigen-presenting cells.
Clone B-H17 monoclonal antibodies have been reported not to inhibit the binding of LFA-1 and do not show reactivity with ICAM-2 and ICAM-3.

Specifications:
Clone B-H17 produces mouse IgG1 antibodies directed against CD54, a 90 - 115 kDa protein. B-H17 was clustered at the Leukocyte Workshop VI (4)

Performance Characteristics:
The CD50 monoclonal antibody, clone B-R1, was analyzed by flow cytometry using blood from a healthy volunteer. Direct staining was performed using 10 ml of FITC conjugated antibody and 100 ml blood sample

Clone B-H17, CD54

Subclass:	IgG1
Source:	mouse
Cellular expression:	haematopoietic and non-haematopoietic cells.
Positive cells:	CD54 FITC 98.4%
Mean fluorescence units:	CD54 FITC 2070.4

References:
1. Smith,M.E.F. and Thomas,J.A., 1990, J. Clin. Pathol. 43. 893-900
2. Springer, T.A., 1990, Nature 346. 425-434
3. Barclay,a.N. et al. 1997, Leucocyte Antigen Facts book. Academic Press. London
4. Leucocyte Typing workshopVI. 1998 Kishimoto et al p.409 ff.
5. Miller,J., et al., 1995, J.Exp.Med. 182. 1231-1241
6. Xu, H. et al. 1994, J.Exp.Med. 180, 95-109

For research only. Not for use in humans. For in vitro use only.