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Product Information CD50
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Introduction:
CD50 or intercellular adhesion molecule-3 (ICAM-3) is constitutively expressed at high levels on leucocytes, including resident epidermal dendritic Langerhans cells (1). It is generally not found on endothelium. CD50 is released from activated lymphocytes and neutrophils, probably by proteolytic cleavage and soluble forms of CD50 are detectable in the blood.
Application:
CD50, clone B-R1, can be applied in biological studies, flow cytometry, or in immuno-histochemistry using cytospots or frozen tissue sections
CD50 plays an important role in providing a costimulatory signal in the immune response and mediates adhesion between leucocytes. It is constitutively expressed on resting antigen-presenting cells, including dendritic cells, and appears to be important in the initial interactions between T cells and cells leading to T cell activation (1). Cross-linking of CD50 on T cells with monoclonal antibodies can mobilize intracellular calcium (2), activate tyrosine phosphorylation, and stimulate T cell adhesion and proliferation (3,4). CD50, like CD54 and CD102 is a ligand for the leucocyte integrin LFA-1 (CD11a/CD18) (5). It is a highly glycosylated type I membrane protein
Specifications:
Clone B-R1 produces mouse IgG1 immunoglobulins directed against a 125 kDa protein known as human CD50. It has been clustered in the Leucocyte Typing workshop VI (6). It reacts with 70 - 100 % of peripheral blood lymphocytes and activated T cells. It is also reactive with a number of human B and T cell lines and human myeloid cell lines. Clone B-R1 also recognizes soluble antigen
Performance Characteristics:
The CD50 monoclonal antibody, clone B-R1, was analyzed by flow cytometry using blood from a healthy volunteer. Direct staining was performed using 10 ml of FITC conjugated antibody and 100 ml blood sample
Clone B-R1, CD50
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Subclass:
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IgG1
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Source:
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mouse
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Cellular expression:
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blood leucocytes, Langerhans cells
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Positive cells:
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CD44 FITC 99.36 %
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Mean fluorescence units:
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CD44 FITC 261.67
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References:
1. Starling, G.C., et al. 1995, Eur. J. Immunol. 25. 2528 - 2532
2. Juan et al. 1994, J. Exp.Med. 179.1747-1756
3. Hernandez-Caselles, T., et al., 1993. Eur.J.Immunol. 23, 2799-2806
4. Barclay ,A.N., et al, 1997. Leucocyte Antigen Facts book. Academic Press. London.
5. de Fougerolles, A.R., et al. 1993. J.Exp.Med. 177. 1187 - 1192
6. Leucocyte Typing workshop VI 1998 Kishimoto et al. p 403 ff.
For research only. Not for use in humans. For in vitro use only.
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