Application:
Monoclonal antiboys CD44, clone MEM-85 can be applied in flow cytometry for analysis of blood and bone marrow samples or in immuno-histochemistry using cytospots or frozen tissue sections. CD44 antigen contributes to the adhesion of leucocytes to endothelial cells, stromal cells and extracellular matrix (ECM). The membrane-distal link module of CD44 binds the ECM GAG hyaluronan (HA). The ability to bind HA is highly regulated in that leucocytes expressing CD44 bind HA only following activation. CD44 appears to be involved in lymphocyte homing to high endothelial venules (HEV) and regulation of LFA-3 adhesion. CD44 is a receptor for the chemotactic cytokine osteopontin

Specifications:
CD44, clone MEM-85 produces mouse IgG2b immunoglobulins directed against the human 85 kDa human adhesion receptor. Clone MEM-85 was clustered at the Leucocyte Typing Workshop IV (3,4).

Performance Characteristics:
CD44, clone MEM-85 monoclonal antibodies were analyzed by flow cytometry using a blood sample from a healthy donor. The cytogram shows direct staining with 10 ml of CD44 FITC and 100 ml of whole blood

Clone MEM-85, CD44

Subclass:	IgG1
Source:	mouse
Cellular expression:	leucocytes (esp. T cells), RBC, bone marrow progenitor cells, brain tissues and epithelial cells.
Positive cells:	CD44 FITC 99.36 %
Mean fluorescence units:	CD44 FITC 261.67

References:
1. Lesley,J., et al 1993. Adv. Immunol. 54. 271-335
2. Barclay, A.N., 1997 The Leucocyte Antigen FactsBook. Academic Press 241-243
3. Knapp,W., et al., eds. 1989 Leucocyte Typing IV. Oxford Univ. Press. Oxford.
4. Bazil,V., Horejsi,V., 1992. J. Immunol. 149. 747-753

For research only. Not for use in humans. For in vitro use only.