Application:
Monoclonal antibody CD41, clone NaM12-6E1, can be applied in flow cytometry for analysis of blood or bone marrow samples or in immuno-histochemistry using cytospots or frozen tissue sections
CD41 antibodies are used for the identification of platelets and the diagnosis of Glanzmann thrombobastemia and leukemia. CD41/CD61 is the major integrin on platelets and is important for platelet adhesion and aggregation. The ligands for CD41/CD61 include fibrinogen, Von Willebrand factor, fibronectin, vitronectin, and thrombospondin (3). Binding to these ligands depends on the activation state of the platelets (1)

Specifications:
CD41, clone NaM12-6E1 produces mouse IgG1 immunoglobulins which bind specifically to platelet glycoprotein complex GP IIb/IIIa (gp 135/gp 110). The CD41 antigen (140 kD) is expressed on platelets and megacaryocytes. Clone NaM12-6E1 was clustered at the Leucocyte Typing Workshop VI (2).

Performance Characteristics:
CD41, clone NaM12-6E1 monoclonal antibodies were analyzed by flow cytometry using a blood sample from a healthy donor. The cytogram shows indirect staining with 10 ml of CD41 followed by anti-mouse FITC conjugate

Clone NaM12-6E1, CD41

Subclass:	IgG1
Source:	mouse
Cellular expression:	platelets and megacaryocytes
Positive cells:	CD41 53.46 %
Mean fluorescence units:	CD41 1767,81

References:
1. Barclay A.N., et al., 1997, The Leukocyte Antigen Factsbook. Academic Press. London.
2. Kishimoto, et al., 1996, Leukocyte Typing VI. Kobe .
3. Phillips, D.R., et al., 1991, Cell 65, 359-362.

For research only. Not for use in humans. For in vitro use only.