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Product Information CD23
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Introduction:
CD23 is a low affinity receptor for IgE (FceR) and both the FceRIIa and FceRIIb are expressed on mature IgD+/IgM+ B cells. The FceRIIb form is also present on monocytes, activated macrophages, eosinophils, platelets and follicular dendritic cells (1). CD23 is also present in a soluble form in serum (2). CD23 together with CD19 and CD22, plays a role in the modulation of B cell activation through membrane immunoglobulin and is involved in regulation of IgE synthesis. Expression of CD23 is increased by IL-4 and down-regulated by B-cell growth factor (BCGF). Binding of IgE immune complexes to CD23 increases the efficiency of B cell antigen processing and presentation. Via its interaction with CD21 it may play a role in adhesion processes. It mediates IgE-dependent cytotoxicity and phagocytosis by macrophages and eosinophils (3,4).
Application:
Monoclonal antibody clone B-G6 (CD23) can be applied in flow cytometry for analysis of blood and bone marrow samples, or in immuno-histochemistry using cytospots or frozen tissue sections.
CD23 is used in studies of B lymphocyte differentiation, and for measurement of Fce receptors in plasma. Most mature IgD+/IgM+ B cells which express CD23, lose this antigen after isotype switching to the downstream isotypes a, g, or e. It is not present on immature bone marrow lymphocytes or T lymphocytes. Its expression is strikingly increased during the B-cell activation process following membranous (m)Ig ligation and IL-4 stimulation or T-B interactions. CD23 binds to the surface adhesion molecules CD11b/CD11c expressed on monocytes and results in the upregulation of pro-inflammatory cytokines such as IL-6, IL-1b and TNF-a (5)
The CD23 antigen is present at low levels on most normal B lymphocytes and is more strongly expressed on activated B cells, Epstein-Barr virus (EBV) transformed lymphoblasts, and chronic lymphocytic leukemia (CLL) cells of B-lymphocyte origin. It is absent in lymphoblastic lymphoma or leukemia, centrocytic lymphoma, hairy cell leukemia and T cell lymphoma.
Specifications:
Clone B-G6 produces mouse IgG1 monoclonal antibodies directed against CD23 antigen. It is suitable for use in flow cytometry and immunohistochemistry
Performance Characteristics:
Staining with CD23 is illustrated by flow cytometry analysis of normal peripheral blood cells with 10ml PE-labeled monoclonal antibody and 100 ml cell suspension. Whole blood was treated with IQ Lyse for lysing of erythrocytes
Clone B-C3, CD19
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Subclass:
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IgG1
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Source:
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mouse
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Cellular expression:
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B cells and monocytes, weak on T cells, FDC, NK cells and others
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Positive cells:
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CD23 PE 9.59 %
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Mean fluorescence units:
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CD23 PE 68.04.
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References:
1. Conrad, D.H., (1990) Annu.Rev. Immunol. 8.623-645
2. Richards, M.L. et al. (1991) Critic.Rev.Immunol. 11.65-86
3. Delespesse, G, et al. (1992) Immunol. rev. 125. 77.
4. Flores-Romo,L, et al. (1993) Science, 2261,1038.
5. Lecoanet-Henchoz, S., et al. (1997) Eur.J.Immunol. 27. 2290-2294
For research only. Not for use in humans. For in vitro use only.
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