Application:
CD106, clone B-K9, can be applied in flow cytometry for analysis of blood or bone marrow samples or in immuno-histochemistry using cytospots or frozen tissue sections.
CD106 antigen binds the integrins a4b1 (CD49d/CD29, VLA-4) and a4b7 (4). VLA-4 is the dominant ligand in cells expressing both integrins. Endothelial CD106 contributes to the extravasation of lymphocytes, monocytes, basophils and eosinophils (but not neutrophils) from blood vessels, especially at sites of inflammation. The VLA-4 interaction can mediate both the initial tethering and rolling of lymphocytes on endothelium as well as their subsequent arrest and firm adhesion (5). CD106 expression on non-vascular tissues is thought to play a role in the interaction of hematopoietic progenitors with bone marrow stromal cells, B cell binding to follicular dendritic cells, co-stimulation of T cells and embryonic development (1,2,3,).

Specifications:
Clone B-K9 produces mouse IgG1 immunoglobulins directed against a 110 kDa protein known as human CD106. It reacts with approximately 15 % of peripheral lymphocytes and between 30 - 90 % activated HUVEC.

Performance Characteristics:
The reactivity of clone B-K9 was analysed by flow cytometry using activated HUVEC activated with TNFa.. Direct staining was performed using 10 ml of FITC conjugated monoclonal antibody and 100 ml cell suspension.

Clone B-K9, CD106

Subclass:	IgG1
Source:	mouse
Cellular expression:	activated endothelium
Positive cells:	CD106 FITC 88.0 %
Mean fluorescence units:	CD106 FITC 394.7

References:
1. Bevilacqua, M.P., 1993. Annu.Rev.Immunol. 11, 767-804.
2. Barclay a.N., et al. The Leucocyte Facts Book. Academic Press. 1997 386-388
3. Carlos,T.M., and Harlan, J.M., 1994. Blood. 84. 2068-2101
4. Berlin,C., et al., Cell. (1993) 74, 185-195
5. Butcher ,E.C., and Picker, L.J., 1996. Science. 272, 60-66

For research only. Not for use in humans. For in vitro use only.