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Product Information CD102
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Introduction:
CD102 antigen, or ICAM-2, is expressed on leucocytes, with the exception of neutrophils, and is
expressed constitutively and at high levels on vascular endothelium (1). It was found and cloned as
the second ligand for lymphocyte-function-associated antigen-1 (LFA-1). Expression is more restricted
than CD54 (ICAM-1).
Application:
CD102, cloneB-T1, can be applied in flow cytometry for analysis of blood or bone marrow samples or
in immuno-histochemistry using cytospots or frozen tissue sections. It also recognizes soluble
antigen and can inhibit cell binding to purified ICAM-2. It partially inhibits ligand binding to LFA-1
(2). It belongs to the Ig superfamily and is thought to play an important role in leucocyte traffic
as an endothelial adhesion receptor and in immune response as a costimulatory molecule (2). Unlike
ICAM-1, induction of CD102 expression with inflammatory mediators is observed little or not at all
(1,3).
CD102 is a highly glycosylated cell surface protein and is a ligand for the leucocyte integrin
CD11a/CD18 (LFA-1).
Specifications:
CD102, Clone B-T1 produces mouse IgG1 monoclonal antibodies which recognize a 55-65 kDa protein.
Clone B-T1 was clustered at the Leucocyte Typing Workshop VI (2).
Performance Characteristics:
Clone B-T1, CD102, was analyzed by flow cytometry using HUVEC activated with TNF-a. Direct staining
was performed using 10 ml of FITC labeled monoclonal antibody.
Clone B-T1, CD102
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Subclass:
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IgG1
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Source:
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mouse
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Cellular expression:
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leucocytes except neutrophils.
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Positive cells:
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CD102 FITC 99.6 %
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Mean fluorescence units:
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CD102 FITC 808.2
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References:
1. de Fougerolles, A.R., et al.. 1991. J.Exp.Med. 174, 253-267
2. Takeuchi,E., et al 1998, in Leucocyte Typing VI Kishimoto et al, p.427.
3. Nortamo,P., et al., 1992, Eur. J. Immunol., 21, 2629-32.
For research only. Not for use in humans. For in vitro use only.
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