(music after F. Liszt)
When cells are in such altered states
You don't know where to set the gates,
It's best to minimize the risk
And store them all on your hard disk.
If there's a clog before you're done,
You'll save some data from a run,
And, thus, you may stay out of jams
You'd get in with live histograms.
List mode, just work in list mode;
When you consider all the options, it's the only thing to do.
This mode, and only this mode,
Lets you make sense of samples which, at first, leave you without a clue.
Once we're in list mode, anyway,
With prices as they are today,
It isn't putting on the Ritz
To digitize to sixteen bits.
It's clear that, once we've made this change,
We'll have enough dynamic range
To transform data digitally,
So log amps will be history.
List mode, we'll work in list mode,
And go from linear to log and back without the log amps' ills.
Once we've got list mode, our only pissed mode
May be when we try pinning down which agencies will pay the bills.
| How many molecules of dyes
And antibodies will be found
On each cell type to which they're bound.
At long last, different labs can see
Results compared objectively,
Advancing science as a whole
And aiding quality control.
List mode, by using list mode,
We'll alt get heightened sensitivities and much reduce the fears
And trepidation of calibration,
Although the folks who make the particles may have us by the spheres.
From East to West, from South to North,
We'll send our data back and forth,
Why, we'll soon have it in our reach
To run our samples from the beach.
But, unless they've been well prepared,
When they are run, we'll run them scared,
List mode or not, there's still no doubt
That garbage in gives garbage out.
List mode, we all need list mode, Though there are ends for which list mode itself can never be the means.
Even with list mode, there won't exist code
Which gets good data from bad samples and/or misaligned machines.
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