CRISP cells are human cell lines that have been prepared by a proprietary method which enables them to remain stable for up to 6 months in the refrigerator.
CRISP cells are stained along with your standard panel to provide a reliable, reproducible positive control.
CRISP cells are provided in 50/50 mixtures of positive and negative cells.
CRISP cells come in handy 10, 30 or 100 test kits.
CD-34
TdT
HLAB27
Illustration of how the CRISP CD-34 positive control cells could be used as an internal control in the assessment of CD-34 positive cells in patient samples.
A normal healthy subject was treated with GCSF. A blood sample was taken and the cell count performed. The sample was spiked with serial dilutions of a know concentration of CRISP CD-34 control cells. The samples were then stained with CD-34 and CD-45 moabs. Using a modified ISHAGE acquisition protocol on the flow cytometer, the CRISP CD-34 control cells are detectable to less than 0.1% of the total cell count. Because the CRISP CD-34 control cells are slightly different in size and fluorescence intensity, they can be gated separately and used as an internal control for patient samples.
