NOTE: Cell fixation using paraformaldehyde is a required step in the
APO-BRDUTM assay. The following cell fixation procedure is a
suggested method. Variables such as cell origin and growth conditions can affect the
results. The fixation conditions provided below should be considered as guidelines.
Additional experimentation may be required to obtain results comparable to the control cells
provide with this kit. The positive and negative control
cells provided in the APO-BRDUTM KIT are already fixed.
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