RiboQuant™ Ribonuclease Protection Assay System

RiboQuant™ T-shirt Special Offer!

Background
PharMingen's new RiboQuant™ Multi-Probe RNase Protection Assay (RPA) system provides a highly specific and sensitive means for detection and quantitation of mRNA species from culture cells or tissues, without further purification of poly-A+ RNA.

RiboQuant Starter Package (cat.#45024K): Includes one Template Set**, the In Vitro Transcription Kit (cat.#45004K) and the RPA Kit (cat.#45014K).

**RiboQuant™ Multi-Probe Template Sets Currently Available Include:

  1. Mouse Cytokine/Chemokine (Available): mCK-1, mCK-1b, mCK-2, mCK-2b, mCK-3, mCK-3b, mCK-4 and mCK-5.
  2. Human Cytokine/Chemokine (Available): hCK-1, hCK-2, hCK-3, hCK-4 and hCK-5.
  3. Rat Cytokine (Available): rCK-1.
  4. Human Apoptosis (Available): hAPO-1, hAPO-2, hAPO-3, hAPO-4 and hAPO-5.
  5. Human Cell Cycle and Tumor Suppressor (Available): hCC-1 and hTS-1.
  6. Rat Neurotrophin (Available): rNT-1.
  7. Mouse Cytokine and Chemokine Receptors (Coming Soon!!): mCR-1, mCR-2, mCR-3, mCR-4, mCR-5 and mCR-6.
  8. Human Cytokine and Chemokine Receptors (Coming Soon!!): hCR-1*, hCR-2, hCR-3, hCR-4, hCR-5, hCR-6 and hCR-7. *Available.
  9. Human Stress (Available): hStress-1.
  10. Mouse Apoptosis (Coming Soon!!!!): mAPO-2 and mAPO-3.


For Research Use Only. Not For Diagnostic or Therapeutic Use.


Mouse Cytokine Template Sets

mCK-1 mCK-1b mCK-2 mCK-2b mCK-3 mCK-3b mCK-4 mCK-5
45001P 45061P 45002P 45051P 45003P 45071P 45025P 45026P
IL-4 IL-4 IL-12p35 IL-12p35 TNF beta TNF beta IL-3 Ltn
IL-5 IL-5 IL-12p40 IL-12p40 LT beta LT beta Il-11 Rantes
IL-10 IL-10 IL-10 IL-10 TNF alpha TNF alpha IL-7 Eotaxin
IL-13 IL-13 IL-1 alpha IL-1 alpha IL-6 IL-6 GM-CSF MIP-1 beta
IL-15 IL-15 IL-1 beta IL-1 beta IFN gamma IFN gamma M-CSF MIP-1 alpha
IL-9 IL-9 IL-1Ra IL-1Ra IFN beta IFN beta G-CSF MIP-2
IL-2 IL-2 MIF IGIF TGF beta1 TGF beta1 LIF IP-10
IL-6 IL-3 IL-6 IL-6 TGF beta2 TGF beta2 IL-6 MCP-1
IFN gamma IFN gamma IFN gamma IFN gamma - TGF beta3 SCF TCA-3
- - - MIF - MIF - -
L32 L32 L32 L32 L32 L32 L32 L32
GAPDH GAPDH GAPDH GAPDH GAPDH GAPDH GAPDH GAPDH


Human and Rat Cytokine Template Sets

hCK-1 hCK-2 hCK-3 hCK-4 hCK-5 rCK-1
45031P 45032P 45033P 45034P 45035P 45027P
- - - - Ltn IL-1 alpha
IL-5 IL-12p35 TNF beta - Rantes IL-1 beta
IL-4 IL-12p40 LT beta IL-3 IP-10 TNF beta
IL-10 IL-10 TNF alpha IL-7 MIP-1 beta IL-3
IL-14 IL-1 alpha IFN gamma GM-CSF Eotaxin IL-4
IL-15 IL-1 beta IFN beta M-CSF MIP-1 alpha IL-5
IL-9 IL-1Ra TGFB3 G-CSF MCP-1 IL-6
IL-2 IL-6 TGFB2 IL-6 IL-8 IL-10
IL-13 - TGFB1 LIF I-309 TNF alpha
IFN gamma IFN gamma - SCF - IL-2
- - - OSM - IFN gamma
L32 L32 L32 L32 L32 L32
GAPDH GAPDH GAPDH GAPDH GAPDH GAPDH


Human Apoptosis Template Sets

hAPO-1 hAPO-2 hAPO-3 hAPO-4 hAPO-5
45111P 45121P 45131P 45141P 45151P
FLICE - FLICE Granzyme A XIAP
Granzyme B bclXL FASL Granzyme B TRAF1
- bclXS FAS DAD1 TRAF2
Mch2 bf1 FADD FAST K CART
- - FAS2L Granzyme H NAIP
ICErel-lll bik FAP RVP1 -
ICH bak FAF Dr-nm23 IAP2
Mch3 bax - Granzyme 3 IAP1
ICE bcl2 TNFRp55 Requiem TRPM2
ICHL - TRADD CAS CRAF (TRAF3)
- mcl1 RIP perforin -
ICE-LAP6 - - - -
L32 L32 L32 L32 L32
GAPDH GAPDH GAPDH GAPDH GAPDH


Human Cell Cycle and Tumor Suppressor / Rat Neurotrophin Template Sets

hCC-1 hTS-1 rNT-1
45081P 45101P 45028P
Cdk1 p130 -
Cdk2 Rb -
Cdk3 p107 betaNGF
Cdk4 DP1 BDNF
p57 DP2 GDNF
p27 E2F1 CNTF
p21 E2F2 NT3
Cdk5 - NT4
Cdk7 E2F4 LIF
PISSLRE - -
p16 E2F5 -
L32 L32 L32
GAPDH GAPDH GAPDH


Mouse Cytokine/Chemokine Template Sets (Coming Soon!!!)

mCR-1 mCR-2 mCR-3 mCR-4 mCR-5 mCR-6
IL-7R alpha IL-5R alpha IL-10R IL-1RI CCR1 -
IL-9R alpha AIC2A IL-11R IL-1RII CCR1b CXCR2
IL-13R alpha GM-CSFR alpha IL-12R beta TNFRp75 CCR3 -
IL-15R alpha IL-3R alpha - TNFRp55 CCR4 CXCR4
IL-4R alpha IL-6R alpha IL-6R alpha IL-6R alpha CCR5 -
gamma c gp130 gp130 gp130 CCR2 BLR-1
IL-2R beta AIC2 beta IFN gammaR alpha TGFBRI - -
IL-2R alpha LIFR alpha IFN gammaR beta TGFBRII - -
- G-CSFR - - - -
L32 L32 L32 L32 L32 L32
GAPDH GAPDH GAPDH GAPDH GAPDH GAPDH


Human Cytokine/Chemokine Template Sets (Coming Soon!!!)

hCR-1* hCR-2 hCR-3 hCR-4 hCR-5 hCR-6 hCR-7
45371P            
IL-7R alpha IL-5R alpha IL-10R IL-1RI CCR1 CXCR1 -
IL-9R alpha IL-3R alpha IL-11R IL-1RII CCR3 CXCR2 -
IL-13R alpha GM-CSFR alpha IL-12R beta TNFRp75 CCR4 CXCR3 CCR5
IL-15R alpha beta c - TNFRp55 CCR5 CXCR4 CCR5(del)
IL-4R alpha IL-6R alpha IL-6R alpha IL-6R alpha TER1 - -
gamma c gp130 gp130 gp130 - BLR-1 -
IL-2R beta LIFR alpha IFN gammaR alpha TGFBRI CCR2a/b BLR-2 -
IL-2R alpha G-CSFR IFN gammaR beta TGFBRII CCR2 alpha V28 -
- - - - CCR2b - -
L32 L32 L32 L32 L32 L32 L32
GAPDH GAPDH GAPDH GAPDH GAPDH GAPDH GAPDH

* Available


Human Stress Template Sets (Now Available)


RiboQuant In Vitro Transcription Kit (cat.#45004K): Reagents and buffers including ribonucleotides, T7 RNA polymerase, and transcription buffer for transcribing anti-sense RNA probes.

RiboQuant RPA Kit (cat.#45014K): Reagents and buffers including hybridization buffer and RNases for RPA analysis.

RiboQuant Multi-Probe Template Sets: Each template sets also includes the appropriate control target RNA (see catalog numbers above for each set).


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