SPECIFICITY
The DX5 antibody reacts with NK cells and a small T-cell subpopulation in all strains tested (e.g., A/J, AKR, BALB/c, C3H/HeJ, C57BL/6, C57BL/10, C57BR, C57L, C58, CBA/Ca, CBA/J, DBA/1, DBA/2, SJL, SWR).(1,2) Multi-parameter flow cytometric analysis has demonstrated that most cells which express NK-1.1 (NKR-P1C), as detected by mAb PK136, also express the DX5 antigen. Small subsets of DX5+ NK-1.1- and DX5- NK-1.1+ are found, especially among the CD3-positive population.(1,3) No activation or blocking activity of the mAb DX5 has been observed.(1)
USAGE
The antibody has been tested by immunofluorescent staining (approx. 1µg/million cells) with flow cytometric analysis to assure specificity and reactivity. Since applications vary, each investigator must determine dilutions appropriate for individual use.
REFERENCES
1. Abrams, J. Personal communication.
2. Moore, T.A., U. von Freeden-Jeffry, R. Murray, and A. Zlotnik. 1996. Inhibition of gd T cell development and early thymocyte maturation in IL-7 -/- mice. J. Immunol. 157: 2366 - 2373.
3. PharMingen. Unpublished results.
| Cat. No. | Description | Clone | Isotype | Size |
| 09941D | Purified anti-mouse Pan-NK Cells | DX5 | Rat IgM | 0.5mg |
| 09944D | FITC labelled anti-mouse Pan-NK Cells | DX5 | Rat IgM | 0.5mg |
PharMingen also supplies a complete line of antibodies to mouse cell surface antigens, including:
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