CellProbe® Reagents
CellProbe Reagents consist of synthetic enzyme substrates optimized to measure intracellular enzyme activity. Generally, enzymatic hydrolysis of the substrate in the cell liberates a fluorescent dye; the amount of dye released is a function of the concentration or activity of the enzyme. The resulting fluorescence can be detected and analyzed by flow cytometry, or any other method of measuring cellular fluorescence. CellProbe Reagents together with flow cytometric analysis comprise the exciting field of Flow Cytoenzymology.
The CellProbe Reagents are substrates that consist of two identical leaving groups conjugated to a dye molecule, i.e., rhodamine-110 (Rho110) or fluorescein.Most of the reagent names contain an abbreviation of the leaving group followed by the name of either the target enzyme(s) or a cell process.
This new technology will enable researchers to measure specific enzyme activity in the natural cellular environment. Suggested research applications include studies of disease processes, effects of drug administration, and cellular processes including activation, apoptosis, differentiation, function, maturation, and proliferation. Previously reported results of enzyme studies often measured total enzyme activity in cell lysates, with altered concentrations of cofactors and contaminating proteases present. Therefore, results of studies utilizing CellProbe Reagents may differ from published research reports.
© Coulter International Corporation, 1996. All rights reserved.
Copyrights and Trademarks
Products are available "For Research use only. Not For Use in Diagnostic Procedures" (RUO). Unless indicated "For In Vitro Diagnostic Use" (IVD).