COULTER™ CD15-RD1
COULTER CD15-RD1 is a monoclonal antibody that detects the CD15 antigen expressed on human neutrophils, eosinophils and monocytes. This antigen is also known as Lewisx (Lex). COULTER CD15-RD1 is used directly from the vial in immunophenotyping protocols. COULTER CD15-RD1 is available only for fundamental research and is not for clinical diagnostic use.
Whole Blood Contour Plot (CD15-RD1)
The light scatter pattern of lysed whole blood populations is readily discernable on a FS vs. LSS plot.
Example Research Areas:Myeloid differentiation/lineage
Lopez, A., Strath M., Sanderson C. Br J Haematol, 1984; 57:489.Reaction with Reed-Sternberg cells and variants
Taatjes, D.J. et al. Am J Clin Path, 1994, 101(2): 140.Studies of CML
Rozman, M. et al. Leukemia, 1993, 7 (1):80.Neutrophil adhesion studies
Stocks, S.C. et al. Biochem J, 1990, 268-275.
Single Parameter Histogram (CD15-RD1)
Gating on the granulocyte population, COULTER CD15-RD1 is analyzed using orange fluorescence (RD1) vs count. An isotypic control is run as part of standard flow cytometric methodology for immunophenotyping. COULTER CD15-RD1 identifies neutrophils and eosinophils.
Molecular Orientation
CD15 antigen is comprised of cell surface glycolipids and glycoproteins with a common terminal pentasaccharide known as Lewisx (3-fucosyl-N-acetyl-lactosamine.) This antigen is found on human white blood cells -- specifically neutrophils, eosinophils and monocytes.
Cell Type
COULTER CD15-RD1 is moderately expressed on monocytes and strongly expressed on neutrophils and eosinophils; it does not react with other white cell populations.
COULTER (TM)
CD15-RD1Specificity
The CD15 antigen is comprised of cell surface glycoproteins and glycolipids with a common terminal pentasaccharide known as Lewisx (Lex). It is expressed by human neutrophils, eosinophils and monocytes.
Product Description
COULTER CD15-RD1 is in liquid form
and is directly conjugated.Concentration: 1µg/10 µL
Vial Contents: 0.5 mL
Isotype: IgM
Part Number: 6607001
Ontogeny
COULTER CD15-RD1 is present on granulocyte-committed cells -- as early as colony forming units and throughout the cell's lifecycle.
For Research Use Only. Not for Use in Diagnostic Procedures.
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