kappa/lambda/CD19 reagents

Eric Hsi (ehsi@luc.edu)
Tue, 31 Dec 1996 12:05:57 -0600

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I have recently discovered this site. I have a question regarding
reagents. We have been testing the Caltag monoclonal kappa/lambda/cd19
antbody cocktail and comparing it to the same BD combination to try and
get a brighter CD19 signal for use in a clinical flow lab. I have
noticed a couple of cases in which the BD reagent shows no evidence of a
light chain restriction but the Caltag reagent does (usually a very low
percentage, dim kappa). Has anyone else seen this? The population
could not be confirmed with polyclonal kappa and lambda from BD or Tago.

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