THP-1's and fluorescent dyes

mckenziekroeger.jl@pg.com
16 Dec 96 18:08:00 -0500

Has anyone worked with the THP-1 cell line using Dihydrorhodamine 123 (DHR)
or Hydroethidine (HE)? I am in search of a method to stimulate these cells
with LPS, an inflammatory cytokine, or virus, and measure oxidative burst.
As of yet, my efforts have come up empty, but I thought there may be some
unpublished or hard to find methods out there in Flowland. Any help,
insights or watch-outs will be appreciated.

Thanks...Jodi McKenzie-Kroeger