Re[2]: apoptosis antibodies (FASL, GRANZ, PERFOR)

tom_frey@bdis.com
Fri, 13 Dec 96 16:53:31

Andreas Christaras

Thanks for providing the information below regarding your experience with anti-
fas-ligand mAbs. However, I had the impression (possibly mistaken) that at
least one of these mAbs recognized an intracellular epitope. Could you clarify
which are able to stain cells without premeabilization?

Tom_Frey@bdis.com

Dear Maria,

following companies provide antibodies against FAS-L (CD95L)

Transduction Laboratories http://translab.com clone 33
in my experience on flow cytometry this mAb works fine
available in purified unlabelled format

PharMingen http://www.pharmingen.com clone NOK-1
this is the antibody I regularly use and it works perfectly
available in purified unlabelled format and biotinylated

PharMingen http://www.pharmingen.com clone NOK-2
I have no experience using this antibody
available in purified unlabelled format

Hope this helps a bit

Andreas Christaras
Heinrich-Heine University Children's Hospital
Laboratory of Experimental Hematology and Stem Cell Transplanatation
KMT-Labor Building 14.82
Moorenstrasse 5
40225 DUESSELDORF
Germany

FON +49-211-81-16103 FAX +49-211-81-16191
E-M christar@uni-duesseldorf.de