cell cycle
Diana Stone (dstone@vetmed.wsu.edu)
Fri, 13 Dec 1996 09:17:37 PST8PDT
We would like to dual label PBMC with PI for cell cycle analysis and with a
FITC-labeled mab to an internal viral protein....both of which require permeablization
of the cell. We have done both procedures seperately, but would appreciate
guidance on how to dual label cells with the PI and the mab for internal
identification of the viral protein. The PBMC will be labeled fresh and after
short term culture (up to 24 hrs.)