I have just joined the list. I'm particularly interested in knowing
other workers interested in flow cytometry of plant material
(probably an enthusiastic minority!). I am currently using a Partec CA-III
to determine DNA amounts in roses.
This is a particularly difficult species viz. phenolics. Does anyone
have experience with contolling phenolics in plant tissue as we have
found they cause a great deal of fluorescence quenching, at least
with DAPI staining.
I am also interested to know if Hoechst dyes have a different
permeability to intact plant cells than does DAPI.
Thanks