enumeration of CD34+ cells

Glenda, M, Davison (GMD@samiot.uct.ac.za)
Fri, 6 Dec 1996 16:26:17 UTC-2

Greetings from Cape Town,

I wonder if anyone out there can help me. I would like to set up a
protocol for accurately quantitating CD34+ stem cells in peripheral
blood stem cell harvests. It seems that most centres are dual
labelling with CD45, but I would like some advice on which protocol
to use as this seems to vary.

I would be very grateful for any advice or any references I could use.

Many thanks in advance,

Glenda Davison
Dept. Haematology
Groote Schuur Hospital
University of Cape Town
email: gmd@samiot.uct.ac.za