Re: G0 versus G1

Kevin G Waddick (waddi002@maroon.tc.umn.edu)
Thu, 5 Dec 1996 11:54:39 -0600 (CST)

I would suggest using staining with Ki-67-FITC (one source being
Immunotech, MIB-1 clone.) Cells in active cycle (G1/S/G2M) would stain
positively, while those cells in G0 would be negative.

Kevin G. Waddick, Ph.D.
Asst. Professor
University of Minnesota
Biotherapy Institute
2625 Patton Road
Roseville, Minnesota 55113
(612) 627-1920 ext. 131 Office
(612) 627-1928 Fax

On Thu, 5 Dec 1996, Eric Miller wrote:

>
> Anyone any pointers to their favourite method of distinguishing G0 cells
> from G1? I have a user here who is trying new compounds on cell lines, and
> showing a decrease in S and G2, along with an increased S phase transit
> time by BRDU labelling. He thinks he may be forcing the cells into G0, but
> I can'y think of the best way to prove this! I have a Facscan, using an HP
> 320 loaded with Cellfit and Lysys II software.
> Thanks in advance.
>
> Eric P Miller
> Edinburgh Medical Oncology Unit
>
> "If the universe is expanding, why can't I find a parking space?"
>