Yes, PE-Cy5 will work fine with FITC and PE as will PerCP. They all have
similar emission maxima at about 670nm, but PerCp may have a slight
advantage as is it not a tandem dye. There are several companies that make
PE-Cyanine conjugates, all giving them different names eg Cychrome
(Pharmingen), TriColor (Caltag), Quantum Red (Sigma) or just PE-Cy5
(Coulter, DAKO). PerCP comes from Becton Dickinson.
As to the range of antibodies available as direct conjugates, Pharmingen,
Caltag and Sigma do a range of anti-human and anti-mouse antibodies while
Coulter and DAKO and BD only do anti-human antibodies (Although I am
prepared to be corrected on this!). Most manufacturers will also do a
streptavidin-conjugated fluorochrome. Your local reps will surely be able
to tell you more!
Hope this is of some help,
Derek
Derek Davies
Imperial Cancer Research Fund, London, UK
http://www.icnet.uk/axp/facs/davies/index.html
On Mon, 2 Dec 1996, Kim Staquet wrote:
>
> Hello,
>
> Continuing on the line of multi-color staining combinations for use with a single laser....
> We want to do triple staining and we have a BD FACScan with an argon laser. Does
> anyone know will PE-cy5 work in combination with FITC and PE? How about PER-CP? If
> so, where can we buy PE-cy5 or PER-CP conjugated goat anti-human or anti-mouse Ig's.
>
> Thanks for your help,
> Kim Staquet
>
> e-mail at Staquetk@Centocor.com
>
>
>
>