Staining with fluorochrome conjugated oligos

Aki Hoji (akhst7+@pitt.edu)
Tue, 26 Nov 96 00:45:28 -0000

Hi, all

Is it possible to stain the cell with fluorochrome conjugated
oligonucleotides that are specific to certain mRNA of the interest.
Also, I have been wondering if it is even possible to achieve multi-color
staining by using biotin conjugated oligos. This approach may be
preferable (i.e. regarding a cost) than using monoclonals in the
situation where the panel of monoclonals must be employed (e.g. TCR
analysis). Any comments or suggestions would be much appreciated.

Happy Thanksgivings,


Aki Hoji (akhst7+@pitt.edu)
Dept. Infectious Diseases and Microbiology
University of Pittsburgh