re: Cryo Preserved Cell Staining

Scott R Burger (burge009@gold.tc.umn.edu)
Fri, 15 Nov 96 15:52:53 -0600

>Anyone with any experience in thawing bags of frozen marrow and keeping
>things alive?
>
>need help
>
>jim houston
>

Sure! We do this in the clinical setting for autologous marrow transplants.
We also do this to obtain hematopoietic progenitor cells and endothelial
cells for more basic research.

Can you describe a bit more specifically what you have in mind? Are there
specific cell populations you want to culture -- stem and progenitor cells,
stromal cells, endothelial cells? How long do want to keep the cells alive?
What will you be doing with the cells?

Scott

***************************************************************
Scott R. Burger, M.D.
Medical Director, Cell Processing Laboratory
University of Minnesota Hospital and Clinic
burge009@gold.tc.umn.edu
612-626-4919
612-624-5411 (Fax)