HLA-DR/CD4 +

Oleg S. Vasilyev (mathmed@bsd.ropnet.ru)
Wed, 13 Nov 1996 22:12:33 -0600

Dear Sirs!

In many of laboratories (by the publications) histograms (flow
cytometry) estimate as percent of positive crates. However, such way of
an estimation histograms too rough, we lose a lot of biological
information. Through mathematical modeling it is possible to receive a
clearer picture of a condition immune system.
Such approach can be applied at exacter diagnostics of a condition
immune system and at study of influence per capita of new medicines.

For the analysis we use histograms with large dispersion
(heterogeneity) such as HLA-DR/CD4 + T-cells and so on (have long "tail"
from active (HLA-DR positive) cells).

On reaction CD4 + T-cells on an antigene we can indirectly judge
reaction immune system and person as a whole on an antigene. In such a
way it is possible to test new medicines.
On financial difficulties we can not now carry out(spend) experimental
work.

If YOU were interested by(with) such approach , can be YOU send
following histograms.
For the given patient with a known pathology or before and after
medicinal therapy (it is unimportant which) are necessary histograms
peripherical blood T-cells:
1. HLA-DR/CD4 +
2. HLA-DR/CD4 + /CD45RA - naiv cells

For an estimation of influence of a given medicinal means it is
necessary to have histograms from several (~20) patients and control
histograms of the donors.

If YOU investigate a given pathology or test a medicinal means, we shall
be glad to give you result of our analysis.

We sincerely hope for further cooperation.

With deep respect
P.G student
Oleg S. Vasilyev
post-graduate student

NATIONAL RESERCH CENTR-INSTITUTE OF IMMUNOLIOGY

RUSSIA, MOSCOW,
Mathmed@ropnet.ru