A researcher here at UCONN is considering using the 413nm violet line
of a Krypton laser to excite mutant GFP. This researcher has been
somewhat dissatisfied with our results using 488nm excitation on the
FACStar Plus (by the way we've been using a 500 EFLP in Fl1 to
capture the emission). Can anyone enlighten me as to the worthiness
of this approach? Is there a new GFP out there or is 413nm excitation
really significantly better?
Gene Pizzo/UCONN Health