I have recently processed several hundred histograms with 'cylchred'.
Some of these have been BrdU +ve/-ve fractions following pulse
labelling. Many of these fit the description above and others have
been more extreme. The program will attempt to find G0/1 G2/M
positions, if you are not happy with these positions values can be
called back from the previous histogram or inserted manually.
Calculation of phases is then based on Watson et al Cytometry 8 1-8
which handles synchronised populations as well as more conventional
distributions.
Version 1.0.5 uses wildcards for easy selection of groups of files
and produces a textfile of results for transferring into
spreadsheets. It is PC based but I have seen it operating on a Mac
with SoftWindows.
Copies from
http://www.cf.ac.uk/uwcm/hg/hoy/
That takes you to my home page which starts with an
illustration from Cylchred
Follow the links to 'Software from Cardiff'
If you prefer ftp
OPEN ftp.cf.ac.uk
CD /pub/UWCM/hg
GET cylchred.exe (as binary).
Terry.
Dr. Terry Hoy
Principal Research Officer
Department of Haematology Telephone 44 1222 743458
University of Wales College of Medicine FAX 44 1222 744655
Heath Park E-mail hoy@cardiff.ac.uk
CARDIFF CF4 4XN http://www.cf.ac.uk/uwcm/hg/hoy/index.html
U.K.