Re: Feotal Heamoglobin

Terry Hoy (Hoy@cardiff.ac.uk)
Thu, 7 Nov 1996 09:34:04 GMT

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>
>Does anybody know of a cocktail that is capable of fixing and
>permeablising adult red blood cells ?
>
>Any comments appreciated.
>
>Andy Johnson, Leeds Blood Transfusion Centre.

You could try the 'fixing and sphering' method developed by Langlois
et al 1990, Cytometry 11 513-521. Details also given in 'Red cells
and reticulocytes' Chapter 7 of 'Flow Cytometry : Clinical
Applications' Ed M.Macey, Blackwell Scientific Publications.
Terry.

Dr. Terry Hoy
Principal Research Officer
Department of Haematology Telephone 44 1222 743458
University of Wales College of Medicine FAX 44 1222 744655
Heath Park E-mail hoy@cardiff.ac.uk
CARDIFF CF4 4XN http://www.cf.ac.uk/uwcm/hg/hoy/index.html
U.K.

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