FITC-PNA

Ronald L. Rabin, M.D. (rr84g@nih.gov)
Wed, 30 Oct 1996 09:25:34 +0800

I have used both FITC and biotin PNA. I found that titrations up to 20
ul/test (test volume about 110 ul) did not saturate, possibly because of
aggregation of the PNA, though I did not pursue this. I would suggest that
you titer on mouse splenocytes, begin with 40 ul/test; you should find that
your resolution is good below saturation, and that yuou will probably
settle on 10 or 20 ul/test.

ron

Ronald L. Rabin, M.D.
Clinical Associate
Cytokine Biology Unit
LCI/NIAID NIH
phone: (301) 402-4910
fax: (301) 496-7383
e-mail: rr84g@nih.gov