the TNF-alpha receptor is sensitive to Trypsin treatment.
Could you use EDTA instead of Trypsin or do fluorescence microscopy with
adherent cells instead of flow for your experiments?
Or could you use another (suspension) cell line to avoid Trypsin treatment
during development of the method?
Good luck
Georg
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H.G. Kreysch
Merck KGaA
Biomed Fo/IMO - A25/410 Tel.: (49)6151 - 72 7323
Frankfurter Str. 250 FAX: (49)6151 - 78 1332
D-64271 Darmstadt E-Mail: kreysch@merck.de