Re: Calcium data analysis

Eric Martz (emartz@microbio.umass.edu)
Thu, 10 Oct 1996 13:29:13 -0500 (EST)

Carolyn D. Dawson recently inquired about calcium analysis software
for HP FACStar + data. MFI has considerable capability in this regard,
is free, and runs on DOS or Windows systems. It can read correctly
nearly all FCS listmode file formats in common use, including HP 310
and 340. MFI can plot a parameter vs. time (if time was recorded in the
listmode file) or against event number (if time was not recorded), i.e.
pseudotime. In addition to a dotplot, MFI generates a line graph of the
median value vs. time or pseudotime. If the listmode file does not include
the desired ratio of two parameters acquired, MFI can calculate the ratio
and use it as an additional parameter. Be sure to get the beta-test
version which has additional capabilities and no 'serious' known bugs. Get
MFI from the comprehensive catalog of free flow cytometry software at
http://www.bio.umass.edu/mcbfacs/flowcat.html

>>Hello Flowers,
>> I would like to know if anyone knows of a program that I may use
>> to analyze calcium data collected on a FacStar plus with a HP computer
>> system. I previously used COTFIT and KINPRO programs on the Vax
>> system. I also have a MAC, but there is know time parameter in the
>> Cellquest software. I would like to be able to retrieve data points,
>> and create a plot. I am currently looking at a demo disk of Multitime.
>> Any suggestions are appreciated.
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Eric Martz, Professor of Immunology emartz@microbio.umass.edu
Molecular visualization (free!): http://www.umass.edu/microbio/rasmol
Flow cytometry (free software!): http://www.bio.umass.edu/mcbfacs
Immunology at UMass/Amherst: http://www.bio.umass.edu/immunology
Dept Microbiology Voice: 413-545-2325 FAX: 413-545-1578
Morrill IVN 203, Box 35720, Univ Massachusetts, Amherst MA 01003-5720
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