Re: Calcium data analysis

Ronald L. Rabin, M.D. (rr84g@nih.gov)
Thu, 10 Oct 1996 09:32:24 +0800

>Hello Flowers,
> I would like to know if anyone knows of a program that I may use to
>analyze calcium data collected on a FacStar plus with a HP computer
>system. I previously used COTFIT and KINPRO programs on the Vax system.
> I also have a MAC, but there is know time parameter in the Cellquest
>software. I would like to be able to retrieve data points, and create
>a plot. I am currently looking at a demo disk of Multitime. Any
>suggestions are appreciated.
>
>
>
>Carolyn D. Dawson
>Centers for Disease Control, MS A-25
>1600 Clifton Road
>Atlanta, GA 30333
>404-639-2721

Cellquest does have a time parameter that can used with ratiometric
analyisis. Are you copying your files as FCS files and using BD's
"FACSconvert?" You may want to give BD a call.

Ronald L. Rabin, M.D.
Clinical Associate
Cytokine Biology Unit
LCI/NIAID NIH
phone: (301) 492-4910
fax: (301) 496-7383
e-mail: rr84g@nih.gov