Re: Dead cells pre-fixation

Alice L. Givan (Alice.L.Givan@Dartmouth.EDU)
08 Oct 96 10:39:07 EDT

Hello Flowers,
With regard to Denis Snider's question about excluding cells that were dead
prior to fixation, there is an interesting paper in Cytometry 19:243-255
(1995) by O'Brien and Bolton --- which uses an anti-tubulin-FITC stain prior to
fixation to mark permeable (i.e. dead) cells.
Alice Givan
Englert Cell Analysis Laboratory
Dartmouth Medical School
Lebanon, NH 03756 USA