Re: your mail

Larry Seamer (LSEAMER@COBRA.UNM.EDU)
Fri, 27 Sep 1996 08:27:37 +0000

I can provide some information concerning the potential pitfalls part of
your question by refering you to a paper by Cinthia Hoy and myself from a
few years ago:

Thermal Denaturation of DNA for Immunochemical Staining of Incorporated
BrdUrd: Critical Factors That Affect the Amount of Fluorescence and the
Shape of BrdUrd/DNA Histogram: Cytometry 10:718-725 (1989).

On -1 xxx -1 SMTP%h82@aixterm1.urz.uni-heidelberg.de@COBRA.UNM.EDU wrote:

> Is anybody of you aware of a good (means working) protocol for
> a Br-desoxyuridine-FACS-procedure? I have used the B-D-protocol
> provided in their monoclonal antibody resource book, but without
> success :-(
> In particular I am looking for information about the FACS-setup
> for aquisition and analysis of cell proliferation and hints
> about possible pitfalls in the procedure.
>
> Any help is greatly appreciated.
>
>
> --
> Andreas Schr=F6der
>
>
>