Re: Phenol red fluoresecence?

Joseph Webster (J.Webster@centenary.usyd.edu.AU)
Wed, 25 Sep 1996 09:08:40 +1100

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At 12:16 PM 24/09/96 EDT, Robin Barclay wrote:
>
>Does anyone know whether phenol red, the common pH indicator in tissue culture
>medium, has any fluorescent properties when associated with cells or any
>quenching properties for other fluorochromes?

I don't know about quenching, but phenol red certainly does fluoresce in
all channels on a FACScan.
At the very least it gives high backgrounds, sometimes makes the readings
completely useless.

Avoid it if possible!

Cheers, Joseph.
Joseph Webster (O.I.C. Flow Cytometry)
Centenary Institute of cancer Medicine & Cell Biology
Locked Bag No.6, Newtown, NSW 2042, AUSTRALIA.
Ph: 61-2-9565-6110 Fax: 61-2-9565-6101

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