Quantitation of 16S Ribosomal Subunit rRNA breaks

Shelton Murinda (sem124@psu.edu)
Thu, 18 Sep 1997 21:34:42 -0500

I understand 16S ribosomal subunit rRNA nucleotide sequences are highly
conserved among all creatures, great (eukaryotes) or small (prokaryotes).
I am planning cytotoxicity studies for quantitating the effect of
ribonucleases (added to the growth medium) on mammalian cells. Is there
any available, quick, cheap and/or facile means of determining specific
cleavage of breaks in the 16S rRNA?

SHELTON E. MURINDA
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Food Microbiology Laboratory
120A Borland Building
Department of Food Science
The Pennsylvania State University
University Park, PA 16802
Phone: 814-862-8475 (H); Phone: 814-863-2970 (W)
Fax: 814-863-6132

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