selection of hybridoma subclones by sorting
Petrie Howard (h-petrie@ski.mskcc.org)
Wed, 17 Sep 1997 16:41:48 -0400
I seem to remember a debate in the past, I can't recall whether it was in
this newsgroup or elsewhere, about the detection and selection of the best
antibody producing clones on the basis of surface immunoglobulin expression
levels, i.e., that in any given line, those cells with the highest density
of surface Ig were (potentially) the best antibody producers. Does anyone
recall this, know of any published data, or have any insights or experience
on this issue? Thanks, HTP
Howard T. Petrie, Ph.D.
Head, Developmental Immunology Laboratory
Director, Monoclonal Antibody Core Facility
Memorial Sloan-Kettering Cancer Center
Box 341, 1275 York Avenue
New York, NY 10021
phone (212)639-2149
fax (212)794-4019