>My question is regarding virus binding to HeLa cells. Briefly, we mix
>virus supernatant with cells for 2h @ 4oC, then come in with a virus-
>specific 1o Ab, followed by a FITC-conjugated 2oAb then FACS analysis
>to differentiate between cells that bound virus from those who didn't.
>My problem is that with HeLa cells, I get a second peak on my
>histograms. People have suggested that perhaps my binding assay damages
>the HeLa cell membrane and allows non-specific incorporation of FITC,
>thus resulting in a peak on the right of the histogram. Does anybody
>have experience in looking at virus binding to HeLa cells and has gotten
>around this problem with possible HeLa cell membrane damage? Also, has
>anybody done LDLR cell surface expression on HeLa cells using FACS?
>Thanks for your help.
>
>Cyril Empig
>
>My email is: empig@genome2.hsc.usc.edu
>
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This message posed by JPR for the above person.
J.Paul Robinson, Ph.D.
Professor of Immunopharmacology
Director, Purdue University Cytometry Laboratories
EMAIL: robinson@flowcyt.cyto.purdue.edu
WEB http://www.cyto.purdue.edu
Phone: (765) 494 0757 FAX (765) 494 0517