NB, thanks to everyone for their suggestions.
Rafael Nunez wrote:
> Dear Flow-ers
> We (Rafael and Claudia) would like to perform some apoptosis assays in
>
> cells that have been stably transfected with an inmediate early gene
> from
> Bovine herpes that is under the control of the tetracyclin operon.
> Therefore, we would like to get more information about how reliable
> are the
> uptake of "Hoechst 33258 and 33342" and "7-AAD" for apoptosis, a few
> days
> ago similar question was asked "Who has expeience using Hoechst uptake
> as
> an indicator of apoptosis?". At the moment, the comments from Miller
> and
> Shapiro seems a little bit controversial, is it not?. We are doing our
>
> homework but your comments will be very much appreciated.
> Thank you for the help.
> Rafael Nunez MD.