Controversy? What controversy? (Was: new kids in apoptosis)

Mark A. Miller (mamiller@biochem.dental.upenn.edu)
Tue, 09 Sep 1997 10:43:09 -0400

If there is any discrepancy between what I wrote and what was written by
any of my esteemed colleagues, especially Dr. Shapiro, I would stick
with whatever THEY wrote. I'll send a subsequent message with all the
replies to my original question.

NB, thanks to everyone for their suggestions.

Rafael Nunez wrote:

> Dear Flow-ers
> We (Rafael and Claudia) would like to perform some apoptosis assays in
>
> cells that have been stably transfected with an inmediate early gene
> from
> Bovine herpes that is under the control of the tetracyclin operon.
> Therefore, we would like to get more information about how reliable
> are the
> uptake of "Hoechst 33258 and 33342" and "7-AAD" for apoptosis, a few
> days
> ago similar question was asked "Who has expeience using Hoechst uptake
> as
> an indicator of apoptosis?". At the moment, the comments from Miller
> and
> Shapiro seems a little bit controversial, is it not?. We are doing our
>
> homework but your comments will be very much appreciated.
> Thank you for the help.
> Rafael Nunez MD.