Re: FITC=//=FITC?

Dave Coder (dcoder@u.washington.edu)
Mon, 8 Sep 1997 14:27:22 -0700

Also relevant, is the observation of the effect of emission difference on
compensation that Joe Trotter illustrated some time back. See the
FITC/Fluorescein Alert! at:

http://facs.scripps.edu/cd4fitc.html

Dave
dcoder@u.washington.edu

>Hi folks,
>While I'm certainly no expert in this area, I feel compelled to
>point out that it is the fluorescence _intensity_ of fluorescein
>that varies with pH; the peak emission wavelength does NOT change. See
>Dr. Haugland's chapter in the latest Methods in Cell Biology manual (part
>B), p 658. I had to rebut this point in response to a reviewer's
>comments recently, which is the only reason I remember it ;-)
>
>I have seen the same spectral difference with an antibody made here on
>campus, but the details of its conjugation were not available. In this
>case I suspect Dr. Prussin is on the right track; it's a matter of
>FITC =//= fluorescein.
>
>Regards,
>Steve
>
>---------------------------------------------------------------
>Steve G. Hilliard steve@habanero.cb.uga.edu
>Cell Analysis Facility, University of Georgia
>If you're a runner you should be dead.
>http://storm.cadcam.iupui.edu/drs/drs.html
>---------------------------------------------------------------
>