I have been attempting to biotinylate a protein which must be
maintained at low pH (around pH3) to retain binding and bio-activity.
The protein denatured in 3M guanidine at neutral pH biotinylates well
but the activity and binding are killed upon refolding. Can anyone
suggest either a biotinylation method or another fluorochrome
conjugation that can be done at low pH with a relatively low
probability of killing my precious protein ?
Steve Neben
Genetics Institute