Re: Radioactive cells on a flow cytometer
J.Paul Robinson (jpr@flowcyt.cyto.purdue.edu)
Thu, 04 Sep 1997 21:31:01 -0500
Regarding the issue of running radioactive cells through the flow cytometer
I have the following comment. We have done this with cells containing low
amounts of tritiated thymidine labelled cells. We were quite unsure about
it, but decided to try it. We took the cells and measured the cpm, ran them
through the cytometer, and then flushed extensively, collecting all the
sheath fluid and measuring successive vials collected. We essentially found
that there was no activiny after just a very short time - the activity was
of course in the cells and once the cells were gone, the instrument was
clear - at least we were unable to measure any activity in the wasted
sheath fluid after a few minutes.
We did subsequent checks and found no activity and concluded that the
proceedure was a reasonable one to perform. The instrument was the Elite.
Hope this helps.
Paul Robinson
>I have a group who wish to do flow cytometric analysis (using a Facscan) on
>cells that have also been treated with an iodinated ligand. The activity is
>"low". I am at the moment rather doubtful about agreeing but I would
>appreciate any comments and advice.
>
>
>Many Thanks
>Pete
>
>
J.Paul Robinson, Ph.D.
Professor of Immunopharmacology
Director, Purdue University Cytometry Laboratories
EMAIL: robinson@flowcyt.cyto.purdue.edu
WEB http://www.cyto.purdue.edu
Phone: (765) 494 0757 FAX (765) 494 0517