DNA analysis buffers

Mark A. Miller (mamiller@biochem.dental.upenn.edu)
Wed, 03 Sep 1997 09:21:18 -0400

I have a few questions about buffers used in DNA content experiments.

1) Why is spermine included in the Vindelov/detergent-based systems?
2) Why do some people use detergents and others use ethanol
permeabilization?
3) How does one make a buffer for eluting low MW DNA out of apoptotic
cells?

Thanks a lot. I love this group!