FITC binding B cells

Walter Sharp (denby@compuserve.com)
Wed, 27 Aug 1997 04:15:22 -0400

Dear All,
Saw a weird one yesterday - a routine 3/4/8/19/45 screen on a
suspected Kala Azar (aka - Leishmaniasis) that appeared to show the norma=
l
B cells picking up FITC conjugates (by one decad more than a normal
specimen) but not PE or PE-CY5.
This occured with IgG1, 2a & 2b classes, whether isotypic or specific.
The bone marrow I ran today showed it was actually a Pre-B leuk which
fitted into the 5% 45weak/19+ cells I picked up in the peripheral screen.=

The main point is - this FITC binding with the (normal & leukaemic) B cel=
ls
had disappeared when processing the BM, probably due to the washing steps=
=2E
Obviously it's something in the plasma of this kid - but what ?
I've never seen anything "specifically" picking up FITC before

Any Ideas

Wal Sharp
SQU
Oman