One Cell Systems WWW Page ? + Microdrop Secretion Assays...

David McFarland (David.McFarland@mcmail.vanderbilt.edu)
Tue, 26 Aug 1997 09:52:41 -0500

I have dabbled in the world of microdrop encapsulation. I can't tell you much
about One Cell Systems though, since I didn't use their equipment. My
experience was through graduate research. We didn't have quite the budget to
lease their microdrop making apparatus. I designed my own apparatus with a
threaded plunger syringe, stir plate, lab jack, and ring stand. This technique
is outlined in:
Gel Microdrop Encapsulation for the Frugal Investigator. In: In Living
Color: Flow Cytometry and Cell Sorting Protocols. Edited by Rochelle Diamond
and Susan DeMaggio. Landes Bioscience Publishers, Georgetown, TX.

Some related articles are:

Kenney JS, Gray F, Ancel M-H, Dunne JF: Production of monoclonal antibodies
using a secretion capture report web. Bio/Technology 13:787-790, 1995.

Powell KT, Weaver JC: Gel microdroplets and flow cytometry: Rapid
determination of antibody secretion by individual cells within a cell
population. Bio/Technology 8:333-337, 1990.

Gray F, Kenney JS, Dunne JF: Secretion capture and report web: Use of affinity
derivatized agarose microdroplets for the selection of hybridoma cells. Journal
of Immunological Methods. 182:155-163, 1995.

Weaver JC, Bliss JG, Harrison GI, Powell KT, Williams GB: Microdrop technology:
A general method for separating cells by function and composition. Methods: A
Companion to Methods in Enzymology 2:3:234-247, 1991.

An alternative to the microdrop encapsulation technique was reported by Rudolf
Manz, et al. I know of at least one lab on this forum that was using this
technique. (Although I don't remember specifically whose lab). This technique
involves direct biotinylation of the cell surface. The reference is:

Manz R, Assenmacher M, Pfluger E, Miltenyi S, Radbruch A: Analysis and sorting
of live cells according to secreted molecules, relocated to a cell-surface
affinity matrix. Proc. Natl. Acad. Sci. 92:1921-1925, 1995.

Happy Hunting!

David McFarland
Vanderbilt Medical Center
Flow Cytometry Facility
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Subject: One Cell Systems WWW Page ? + Microdrop Secretion Assays...
From: Matthias Haury <mhaury@pasteur.fr> at +inet
Date: 08/25/1997 9:08 PM

Hello again,

I have a couple of questions from a friend concerning the CellSys Agarose
Microdrop maker, sold by One Cell Systems, Cambridge MA.

1. Does anybody have the WWW adress, email, tel-fax no etc of "One Cell
Systems"?

2. Are there people out there who have used cytokine secretion capture with
agarose microdrops with good results ? The idea is to capture the secreted
cytokines in the agarose and detect them with flow cytometry. Preferably in
living cells, no fixation... cells must be sorted on cytokine secretion and
then be still clonable...

Any hints, references etc would be appreciated as well.

Thanx a lot in advance.

Matthias

_____________________________________________________________________________
Matthias Haury Flowcytometry Dept Immunology Institut Pasteur
mhaury@pasteur.fr Tel: 33 (01) 40 61 31 29 Fax: 33 (01) 45 68 86 39
_____________________________________________________________________________