Antibody removal
Kevin Holmes (KHOLMES@atlas.niaid.nih.gov)
Mon, 25 Aug 1997 16:40:02 -0400
An investigator in our Institute asked me a question that I'd like to
pass along:
He is doing a multicolor sorting experiment and wants to remove the
fluorochrome labeled antibody after sorting, before he puts the cells
into culture.
The only thing I could think of was trypsin treatment, but I was
unsure of the long term effects of this, or whether this would cause
more harm than good.
Any suggestions?
Thanks.
Kevin L. Holmes, Ph.D.
Head, Flow cytometry Unit
Office of the Scientific Director
Bldg 7, Room 01
NIAID, NIH
Phone: 301-496-9071
FAX: 301-402-4532
Email: kholmes@atlas.niaid.nih.gov