Re: NK leukemia?

Anna Porwit-MacDonald (anpo@mb.ks.se)
Mon, 25 Aug 1997 13:14:41 +0200

Dear Rafael,
Thank you very much for your comments on the NK-cell leukemia case.
We have now got a new cytogenetics (at the time of progress) which showed
after 24h culture a clone: 45X,-Y,add(2),-7,i(7)(q10),add(8),-16+mar[12],
which is consistent with a malignant clone.
We have cells frozen both for DNA/RNA and in liquid nitrogen, but not much.
The studies you suggested are very interesting indeed, so when we thaw the
cells I will check the IFN staining by flow and I will discuss the
possibility of establishing the cell line with the research lab.
(unfortunately I do not have such possibilities at Hematopathology). I will
also talk to clinicians about checking IL-12.
The patient started the high-risk ALL protocol last week.
Best wishes
Anna
Anna Porwit-MacDonald,MD,PhD
Haematopathology Lab.,
Karolinska Hospital, Stockholm

>To
>Anna Porwit-MacDonald
>>Haematopathology Lab.,
>>Karolinska Hospital, Stockholm
>and the others Flowers
>Anna
>If I recall right, you have posted a case about a patient with a likely
>leukemia of NK cells, dont you?.
>I have some questions relate to this topic. Have you check if the cells
>produce Interferon ? by using intracytoplasmic staining and FACS (finally
>a FACS procedure). How are IL-12 levels of the patient?. Can you keep the
>cells in culture?, having cell lines of this patient would be great. (I
>would love to have one split of the cells for...) My guess is if that
>leukemia is a NK cell, you should be able to detect high IL-12 (very, very
>high) as well as interferon gamma.
>Regards and keep the good job
>Rafael
>
>
> \|/
> (o o)
>________________________________oOo__(_)__oOo_________________________________
> ___/\_ | Rafael Nunez mailto:rafaeln@vetvir.unizh.ch
> / o \/| | University Inst.for Virology http://www.unizh.ch/vetvir
> / _| | Winterthurerstr. 266a Telephone: (+41) 1 6358709
> /_/\__/-\/ | 8057 Zurich SWITZERLAND Faximile : (+41) 1 6358911
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