Re: Human FcR (CD16, CD32, CD64)
Pilar Calo (calomata@facstaff.wisc.edu)
Thu, 21 Aug 1997 12:00:55 -0500
On Wed 13 Aug, R Barclay wrote:
>
> Can anyone suggest reliable sources of antibodies for studies of these FcR
> markers on human leucocytes?
> Also - has anyone any experience of looking at these in a whole blood (lyse
> and wash) method - and are there any pitfalls to be avoided? What blocking
> agents are commonly employed (rather not use gammaglobulin)?
> Any advice or dialogue welcome.
> Robin Barclay
> SNBTS, Edinburgh
>
>
Medarex supply antibodies to all three receptors and if you use antibodies
which are outside of the IgG binding site you shouldn't have a problem with
competition from plasma IgG.
Mike Clark, <URL:http://www.path.cam.ac.uk/~mrc7/>
--
o/ \\ // || ,_ o M.R. Clark, PhD. Division of Immunology
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