RBC Lysis

Moss, Delynn M. (dmm3@cdc.gov)
Mon, 18 Aug 1997 13:18:00 -0400

Recently, I have observed trypan blue uptake by lymphocytes from murine
messenteric lymph nodes that have been exposed to 0.14 M NH4Cl buffered
with 0.017 M Tris pH 7.2 for 5 minutes at 4 degress C. Is there some
other RBC lysing solution that is preferable over NH4Cl/Tris solutions
that will not harm lymphocytes?

Thanks

Delynn Moss
Parasitic Diseases Division
Centers for Disease Control